Coding

Part:BBa_K3898152:Design

Designed by: Yuliang Huo   Group: iGEM21_DUT_China   (2021-10-01)


RIDD-PETase：optimized PETase with RIDD (comparing with BBa_K2010999)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 360
    Illegal AgeI site found at 74
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimized for E. coli BL21 using the Codon Optimization Tool from the genscript website.

Source

R0184, J61100, K3898001, K3898003, B0012

References